Demystifying the Hill Equation for Assay Developers
The Hill-model and similar ones are often used in the fields of drug discovery to model experimental data from dose-response-, binding- or bioassays. In this blog post I will try…
The Savitzky-Golay Method: Smoothing and Differentiating Experimental Data
The first time I came across smoothing with the Savitzky-Golay method was during my chemometrics class at university analyzing spectroscopy data using chemoetric methods. And indeed, the Savitzky-Golay method seems…
Finding roots and Hunting for an Optimum
In process control it is a common goal to maximize the yield of a product by finding the optimal process parameters. In a bioreactor for instance, parameters like temperature, nutritional…
Bacterial density
Bacteria and Their Most Probable Mumber (MPN)
Analyzing drinking water or food for viable bacteria and other microbes is important to prevent epidemic infections. In practice it can be difficult to count the number of viable bacteria…
Bioassay performed in a microplate
Analysis of Bioassays
Recently, I went through the woods with one of my younger kids and it was amazed by the various colors of different mushrooms. Kids tend to touch almost everything and…
Weighted Regression — Is it cheating?
Linear and non-linear regression are key tools that scientist apply regularly to model their data. This ranges from setting up a linear calibration line up to modelling biochemical processes like…
How to use Excel’s LAMBDA Function for Data Analysis?
Recently, Microsoft introduced the so-called LAMBDA function allowing users to define custom Excel functions. The concept of user-defined functions in Excel is not new. However, in the past these had…
Logistic regression in vaccine testing
I guess almost every scientist is familiar with linear regression and has already spent quite some time using it for various assays like BCA or Bradford assay for protein concentration…
Data Analysis Book — fresh from the press
After 3.5 years of continous work, 3.5 years of lack of sleep and 3.5 years apologizing to my kids and my wife for spending too less time with them, my…
Most European countries did not show exceptional excess mortalities during SARS-CoV-2 pandemic
Currently many political discussions and decisions are dominated by new increasing SARS-CoV-2 cases. However, as these numbers are dependent on the precision of the corresponding tests it is wise to…